Picture time

End of the experiments, the write ups begin

Last week I finished all my lab work :D however this means I have 2 whole weeks to write up stuff and make presentations. So last week I tested using really small volumes (80ul) and it worked, sort of. Now all I need to do is suggest some improvements, no pressure then. I have soo much writing to do I'm glad I have these two weeks, and two presentations and one is infront of the people in the lab so they actually know what I'm talking about, gulp!

End of the protein prep :)

Hopefully today I will have purified some S1 from myosin. It has been a busy week making very large quantaties of buffer (25L is my record) and as of this morning when I assayed the solution I calculated the total mass of the protein to be a massive, an incredible 1.36 g. Apparently this is good, but I can't help but feel its a little small as I started of with aroung 750ml of myosin. But anyway get to fast freeze it in liquid nitrogen later, very cold liquids and me may be a dangerous mix but hey better give it a go. I am honestly happy that my protein no longer looks like a white, sticky, mush which being a sick minded individual made me think of... never mind. It is dawning on me that I only have 3 weeks left, which is a little scary. Still I'm sure I will have 3 more fun filled weeks of stoppe-flow :D